normal cell lines a549  (ATCC)

Journal: Discover Oncology

Article Title: PEITC restores chemosensitivity in cisplatin-resistant non-small cell lung cancer by targeting c-Myc/miR-424-5p

doi: 10.1007/s12672-025-03824-1

Figure Lengend Snippet: Effect of different concentrations of cisplatin on the viability of A549 and the A549/DDP cells at 48 h ( A ), the effect of different concentrations of cisplatin on the viability of PC-9 and the PC-9/DDP cells at 48 h ( B ), effect of different concentrations of PEITC on the viability of the A549/DDP and the PC-9/DDP cells at 48 h ( C ), and effect of PEITC concentration of the A549/DDP cell line in the presence of 38 µM cisplatin (IC50) on cell viability from 12 to 72 h ( D ). CI isobologram of A549/DDP cells with 8 µM PEITC and 15, 30, 45, 60, 75, 90 µM cisplatin ( E ). CI isobologram of the PC-9/DDP cells with 8 µM PEITC and 10, 20, 30, 40, 50, 60 µM cisplatin ( F ). Flow cytometry analysis of the effect of different concentrations of PEITC in the presence of 38 µM cisplatin (IC50) on the percentage of apoptotic the A549/DDP cells at 24 h ( G ) and on cell cycle progression (H). ****, P < 0.0001

Article Snippet: Normal A549 NSCLC adenocarcinoma cell line was obtained from Procell (China), a cisplatin-resistant line of NSCLC cells (A549/DDP) was obtained from Procell (China), and a PC-9 NSCLC adenocarcinoma cell line was obtained from Zhongqiaoxinzhou (China).

Techniques: Concentration Assay, Flow Cytometry

Journal: Discover Oncology

Article Title: PEITC restores chemosensitivity in cisplatin-resistant non-small cell lung cancer by targeting c-Myc/miR-424-5p

doi: 10.1007/s12672-025-03824-1

Figure Lengend Snippet: Effect of 38 µM cisplatin alone and in the presence of 46 µM PEITC on expression of genes in the PI3K/AKT/mTOR pathway based on western blotting ( A ) and qRT-PCR ( B ), and expression of SCOS5 and SCOS6 based on western blotting ( C ) and qRT-PCR ( D ) in A549/DDP cells at 48 h. **, P < 0.01; ****, P < 0.0001

Article Snippet: Normal A549 NSCLC adenocarcinoma cell line was obtained from Procell (China), a cisplatin-resistant line of NSCLC cells (A549/DDP) was obtained from Procell (China), and a PC-9 NSCLC adenocarcinoma cell line was obtained from Zhongqiaoxinzhou (China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Discover Oncology

Article Title: PEITC restores chemosensitivity in cisplatin-resistant non-small cell lung cancer by targeting c-Myc/miR-424-5p

doi: 10.1007/s12672-025-03824-1

Figure Lengend Snippet: Effect of 38 µM cisplatin alone and 46 µM PEITC on expression of miR-424-5p (A, qRT-PCR) and c-Myc (B, qRT-PCR and western blotting) in A549/DDP cells at 48 h. ***, P < 0.001; ****, P < 0.0001

Article Snippet: Normal A549 NSCLC adenocarcinoma cell line was obtained from Procell (China), a cisplatin-resistant line of NSCLC cells (A549/DDP) was obtained from Procell (China), and a PC-9 NSCLC adenocarcinoma cell line was obtained from Zhongqiaoxinzhou (China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Discover Oncology

Article Title: PEITC restores chemosensitivity in cisplatin-resistant non-small cell lung cancer by targeting c-Myc/miR-424-5p

doi: 10.1007/s12672-025-03824-1

Figure Lengend Snippet: Identification of the 3′UTR of c-Myc as a target of miR-424 based on TargetScan ( A ) and a dual-luciferase reporter assay ( B ). Effect of a c-Myc silencing vector in A549/DDP cells on expression of c-Myc ( C ), expression of miR-424-5p ( D ), and expression of SOCS5, SOCS6, AKT, p-AKY, PI3K, and p-PI3K ( E ) after 48 h. con control (no transfection), NE-OV transfection with an empty vector; si–c-Myc transfection with a silencing vector

Article Snippet: Normal A549 NSCLC adenocarcinoma cell line was obtained from Procell (China), a cisplatin-resistant line of NSCLC cells (A549/DDP) was obtained from Procell (China), and a PC-9 NSCLC adenocarcinoma cell line was obtained from Zhongqiaoxinzhou (China).

Techniques: Luciferase, Reporter Assay, Plasmid Preparation, Expressing, Control, Transfection

Journal: Discover Oncology

Article Title: PEITC restores chemosensitivity in cisplatin-resistant non-small cell lung cancer by targeting c-Myc/miR-424-5p

doi: 10.1007/s12672-025-03824-1

Figure Lengend Snippet: Effect of 46 µM PEITC on A549/DDP cells that overexpressed c-Myc on cell cycle progression ( A ), apoptosis ( B ), expression of miR-424-5p ( C ) expression of c-Myc ( D ), and expression of AKT, p-AKT, PI3K, p-PI3K, SOCS5, and SOCS6 ( E , F ) after 24 h

Article Snippet: Normal A549 NSCLC adenocarcinoma cell line was obtained from Procell (China), a cisplatin-resistant line of NSCLC cells (A549/DDP) was obtained from Procell (China), and a PC-9 NSCLC adenocarcinoma cell line was obtained from Zhongqiaoxinzhou (China).

Techniques: Expressing

Journal: Thoracic Cancer

Article Title: Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF ‐β1

doi: 10.1111/1759-7714.15495

Figure Lengend Snippet: PVRL4 silencing suppresses LUAD cell proliferation, invasion, and migration and induces cell apoptosis. (A‐G) The sh‐PVRL4 or sh‐NC was transfected into A549 and HCC827 cells. (A) Western blotting for PVRL4 levels in cells. (B‐D) CCK‐8 and colony formation assays for cell proliferation. (E) Transwell for cell invasion. (F) Wound healing assay for cell migration. (G) Flow cytometry analysis for cell apoptosis. * p < 0.05.

Article Snippet: LUAD cell lines A549 and HCC827 and the normal 16HBE cells were purchased from Procell (Wuhan, China) and then cultured in RPMI‐1640 medium (PM150110) plus1% penicillin/streptomycin (PB180120) and 10% FBS (164210‐50) (Procell) at 37°C with 5% CO 2 .

Techniques: Migration, Transfection, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Thoracic Cancer

Article Title: Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF ‐β1

doi: 10.1111/1759-7714.15495

Figure Lengend Snippet: PVRL4 is transferred into PBMCs from LUAD cells by exosomes. (A) TEM analysis for exosome morphology. (B) Western blotting analysis for exosomal markers (CD9, CD63 and Alix). (C) The uptake of A549‐Exo and HCC827‐Exo was observed using the PKH67 staining. (D, E) Levels of PVRL4 protein were detected by western blotting in PMSCs after incubating with A549‐Exo, HCC827‐Exo or PBS (Control). (F, G) PBMCs were incubated with PBS, A549 or HCC827 cells and PBS, or A549 or HCC827 cells and GW4869, and levels of PVRL4 protein were examined by western blotting in PMSCs. * p < 0.05.

Article Snippet: LUAD cell lines A549 and HCC827 and the normal 16HBE cells were purchased from Procell (Wuhan, China) and then cultured in RPMI‐1640 medium (PM150110) plus1% penicillin/streptomycin (PB180120) and 10% FBS (164210‐50) (Procell) at 37°C with 5% CO 2 .

Techniques: Western Blot, Staining, Control, Incubation

Journal: Thoracic Cancer

Article Title: Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF ‐β1

doi: 10.1111/1759-7714.15495

Figure Lengend Snippet: PVRL4 knockdown in LUAD cell exosomes suppresses MDSC induction and the level of MDSC‐secreted TGF‐β1. (A‐G) PBMCs were incubated with PBS, HCC827‐Exo sh–NC , A549‐Exo sh–NC , HCC827‐Exo sh–PVRL4 , or A549‐Exo sh–PVRL4 . (A) Levels of PVRL4 were detected by western blotting. (B, C) Flow cytometry for the number of CD14 + HLA‐DR − MDSCs after co‐incubation. (D‐G) ELISA analysis and western blotting for TGF‐β1 levels in PBMCs after co‐incubation. * p < 0.05.

Article Snippet: LUAD cell lines A549 and HCC827 and the normal 16HBE cells were purchased from Procell (Wuhan, China) and then cultured in RPMI‐1640 medium (PM150110) plus1% penicillin/streptomycin (PB180120) and 10% FBS (164210‐50) (Procell) at 37°C with 5% CO 2 .

Techniques: Knockdown, Incubation, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Thoracic Cancer

Article Title: Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF ‐β1

doi: 10.1111/1759-7714.15495

Figure Lengend Snippet: Knockdown of exosomal PVRL4 suppresses LUAD cell proliferation, invasion, and migration and induces cell apoptosis by regulating MDSC‐secreted TGF‐β1. (A‐K) A549 and HCC827 cells were incubated with TGF‐β1 overexpressed MDSCs, followed by incubating with HCC827‐Exo sh–NC , A549‐Exo sh–NC , HCC827‐Exo sh–PVRL4 , or A549‐Exo sh–PVRL4 . (A) Levels of PVRL4 were detected by western blotting. (B–E) CCK‐8 and colony formation assays for cell proliferation. (F, G) Transwell for cell invasion. (H, I) Wound healing assay for cell migration. (J, K) Flow cytometry analysis for cell apoptosis. * p < 0.05.

Article Snippet: LUAD cell lines A549 and HCC827 and the normal 16HBE cells were purchased from Procell (Wuhan, China) and then cultured in RPMI‐1640 medium (PM150110) plus1% penicillin/streptomycin (PB180120) and 10% FBS (164210‐50) (Procell) at 37°C with 5% CO 2 .

Techniques: Knockdown, Migration, Incubation, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Antiviral research

Article Title: Influenza virus NS1- C/EBPβ gene regulatory complex inhibits RIG-I transcription

doi: 10.1016/j.antiviral.2020.104747

Figure Lengend Snippet: Heat map of differentially expressed genes based on PCR Array analysis. NHBE cells were transfected with vector, hC/EBPβ, control siRNA or hC/EBPβ siRNA for 24h. Cells were then mock infected or infected with PR8 (1.0 MOI) for 6 h. Gene expression were analysed by real-time RT-PCR using PCR array kit as per manufacturer protocol. Data shown are average from two independent experiments. The scale shows the level of gene expression where red corresponds to upregulation (log2 fold).

Article Snippet: Human lung epithelial cell line A549, and normal human bronchial epithelial (NHBE) cells (Lonza, Switzerland) were maintained as described ( Ranjan et al., 2010 ).

Techniques: Transfection, Plasmid Preparation, Control, Infection, Gene Expression, Quantitative RT-PCR